In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens

BLAJMAN, JÉSICA; GAZIANO, CRISTIAN; ZBRUN, MARÍA; SOTO, LORENA; ASTESANA, DIEGO; BERISVIL, AYELÉN PATRICIA; ROMERO SCHARPEN, ANALÍA; SIGNORINI, MARCELO; FRIZZO, LAUREANO

RESEARCH IN VETERINARY SCIENCE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2015 vol. 101 p. 50 - 56

0034-5288

Among 360 isolates from thegastrointestinal tract (GIT) of broilers, eleven isolates which showed in vitroprobiotic propertieswere identified and selected for further tests. After thein vitro screening, three strains were chosen for the in vivo study ofpersistence of fresh cultures and then one strain was selected for the in vivostudy of persistence of lyophilized culture. Lyophilized Lactobacillussalivarius DSPV 001P was capable of persisting in broilers during a completerearing, even 28 days following cessation of administration. L. salivarius DSPV001P administered to broilers and recovered from GIT was compared bypulsed-field gel electrophoresis (PFGE) to ensure that the same genotype waspersistently identified. A combination of in vitro and in vivo screening ofnative lactic acid bacteria (LAB) described in this study may offer a methodfor selecting the most suitable strain for potential application as a broilerprobiotic supplement.