Pathogen translocation and histopathological lesions in an experimental model of Salmonella Dublin infection in calves supplemented with lactic acid bacteria and lactose

FRIZZO, LAUREANO; ZBRUN, MARÍA; SOTO, LORENA; BERTOZZI, EZEQUIEL; SEQUEIRA, GABRIEL; MARTÍ, LUIS; SIGNORINI, MARCELO; RODRÍGUEZ ARMESTO, ROBERTO; ROSMINI, MARCELO

JOURNAL OF VETERINARY SCIENCE

KOREAN SOC VETERINARY SCIENCE

Lugar: Seoul; Año: 2012 vol. 13 p. 1 - 13

1229-845X

The aim of this work was to evaluate the capacity of a microbial inoculum composed of three lactic acid bacteria strains to protect young calves supplemented with lactose against Salmonella Dublin infection by studying histopathological lesions and pathogen translocation. Fifteen calves divided into three groups (control group, C-G; group inoculated with LAB, LAB-G; and group inoculated with LAB and lactose, L-LAB-G), with five, six and four animals each, respectively, were used. The inoculum, composed of Lactobacillus casei DSPV 318T, L. salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T, was administered together with milk replacer. The LAB-G and L-LAB-G received a daily dose of 109 CFU/kg body weight of each strain throughout the experiment. Lactose was provided to the L-LAB-G in doses of 100 g/d. Pathogen was orally administered to all animals on day 11 of experiment with 2x1010CFU. Microscopic lesion index in target organs was 83%, 70% and 64.3% (P<0.05) for the C-G, LAB-G and L-LAB-G, respectively. The administration of probiotic inoculum was not fully effective for fighting infection caused by Salmonella. Although probiotic treatment was unable to delay the arrival of pathogen to target organs, it was evident that the inoculum altered the response of animals to pathogen attack.