Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of Fusarium graminearum

LEONEL MAXIMILIANO ORTEGA; MARÍA CANDELA MOURE; ESTEBAN MANUEL GONZÁLEZ; TERESA MARIA ALCONADA

INTERNATIONAL MICROBIOLOGY

VIGUERA EDITORES

Lugar: Barcelona; Año: 2019 vol. 22 p. 289 - 296

1139-6709

Wheat gluten proteins are decisive for the industrial properties of flour, so alterations resulting from grains infection with Fusarium graminearum, produce changes in the glutenin content that affect the baking properties. This work analyzes the high molecular weight glutenin changes from wheat flour with different degree of F. graminearum infection at field, since these proteins are determinant for the quality properties of flour. Wheat cultivars ?on field trials? infected with F. graminearum isolates of diverse aggressiveness, showed severity values between 9.1?42.58% and thousand kernel weight values between 28.12?32.33g. Negative correlations between severity and protein content and positive correlations between yield and protein content were observed, employing reverse phase high performance liquid chromatography and polyacrylamide gel electrophoresis. Furthermore, the protein signal changes were in agreement for both methodological approaches. Also, the degree of disease observed and the protein changes on infected wheat cultivars varied in relation with the aggressiveness of the isolate responsible for the infection. The principal components analysis showed a close arrangement among protein values obtained by HPLC. For each cultivar, two principal components were obtained, which explained 80.85%, 88.48% and 93.33% of the total variance (cultivars Sy200, AGP Fast and Klein Tigre respectively). To our knowledge, the approaches employed for the analysis of protein changes according to the degree of disease, as well as the thorough statistical analysis, are novel for the study of Fusarium Head Blight.