Pectic activities from Fusarium oxysporum f.sp.melonis. Purification and Characterization of an exopolygalacturonase.

MARTÍNEZ, MARÍA JESÚS; ALCONADA TERESA; GUILLÉN FRANCISCO; VÁZQUEZ COVADONGA; REYES FUENSANTA

FEMS MICROBIOLOGY LETTERS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 1991 vol. 81 p. 141 - 150

0378-1097

Fusarium oxysporum f. sp. melonis produced an extracellular enzymic mixture with high pectic activities, (at least an exopolygalacturonase, an endopolygalacturonase and two lyases) in a medium with glucose and pectin as carbon sources. An exopolygalacturonase from this crude enzyme preparation was purified 23.8 times by Sephadex G-200 and ion-exchange HPLC. It had a K m of 6 mM, a M r of 58000, apI of 6.4, an optimum pH of 5 and was stable in the 3.5-6.5 pH range. This enzyme preferentially hydrolysed polygalacturonic acid, showing only 5% activity on pectin, and did not exhibit the activity of an endoenzyme.