INVESTIGADORES
TARGOVNIK Alexandra Marisa
artículos
Título:
Simple production of hydrophobin-fused domain III of dengue envelope protein and induction of neutralizing antibodies against the homotypic serotype of dengue virus
Autor/es:
JULIETA CEREZO; ALEXANDRA M. TARGOVNIK; MARÍA EMILIA SMITH; DANIEL GONZALEZ MAGLIO; VICTORIA CELINA LUPO; MARÍA ALEJANDRA MORALES; MARÍA VICTORIA MIRANDA; JULIAN RODRIGUES TALOU
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2020
ISSN:
0141-5492
Resumen:
Hydrophobin-fused Domain III of dengue envelope proteins serotypes 1 and 2 wereexpressed in Rachiplusia nu larvae and purified by aqueous two-phase system. Thisbiotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7μg/larva of fusion protein DomIII serotype 1 and 61.4 μg/larva of fusion protein DomIIIserotype 2, represents an integrated strategy for simple production of recombinantantigens. Purified fusion proteins induced serotype specific neutralizing antibodieswithout cross reaction against other serotypes and arboviruses after mouseimmunization. Hydrophobin-fused Domain III of dengue envelope protein could be apromising strategy for easy and low-cost production of components of a tetravalentsub-unit vaccine against dengue.
