Biocompatible supramolecular platforms to modulate cell adhesion

PASQUALE, MIGUEL A.; MUZZIO, NICOLÁS E.; MARMISOLLE, WALDEMAR; VON BILDERLING CATALINA; CORTEZ, M. LORENA; PIETRASANTA, LÍA I.; AZZARONI, OMAR

La PLata

Congreso; Reunión Anual SAB; 2018

Sociedad Argentina de Biofísica

Cell adhesion modulation via biologically inspired materials plays a key role in thedevelopment of realistic platforms to envisage mechanistic descriptions of manyphysiological and pathological processes as well as the design of biomedicaldevices.This contribution show the control of cell adhesion and proliferation of threedifferent cell lines employing a novel biologically inspired supramolecular coatinggenerated via dip coating of the working substrates in an aqueous solution ofpolyallylamine in the presence of phosphate anions in one-step procedure.Results reveal that selective cell adhesion can be controlled by varying thedeposition time of the coating. Cell proliferation experiments showed a cell typedependent quasi-exponential growth demonstrating the nontoxic properties of thesupramolecular platform. From a certain deposition time, the supramolecular filmsdisplayed antiadhesive activity towards cells, irrespective of the cell type.However, these antiadherent substrates recover good adhesive properties afterthermal annealing. These results were interpreted based on the changes in thecoating hydrophilicity, topography and stiffness. This material may have useseither to improve cell adhesion or as an antifouling surface. The former would beachieved employing thin coatings or annealed ones and antifouling propertieswould be obtained for thicker unannealed coatings. The proposed approach issimple and flexible, and offers opportunities for the development of tunable,biocompatible architectures to control cell phenotype of interest for biomedicalapplications.