DE NOVO ASSEMBLY AND CHARACTERI ZATI ON OF ROOT TRANSCRIPTOME OF Ilex paraguariensis ( YERBA MATE) UNDER DROUGH ST RESS.

AVICO, EDGARDO H.; ACEVEDO, RAÚL M.; CALZADILLA, PABLO; RUIZ, OSCAR A.; SANSBERRO, PEDRO A.

Corrientes

Congreso; XXXI Reunión Argentina de Fisiología Vegetal; 2016

Sociedad Argentina de Fisiología Vegetal

The root system is essential for monitoring soil water status.The biochemical processes triggered in this organ during a period of drought, alert the rest of the organism and maximize water uptake. In order to understand the molecular basis of these responses in roots of yerba mate, root transcriptome sequencing was performed in contrasting conditions of soil water availability (ψsoil = ~ - 0. 04 MPa and - 2 MPa). Illumina1500 Hi Seq platform was used, followed by de novo assembly. A total of 132,387 unigenes and 222, 232 transcripts were obtained from 172,128,258 pair- ends reads ( 2x150bp) from 6 libraries (control and stress sample triplicated). Among them, 49% could befunctionally annotated using public databases.The comparative analysi s of transcriptomes revealed that 1,189 transcripts were differentially expressed ( FDR |2|),of which 533 were up-regulated and 656 downregulated by water stress. Functional enrichments using Blast 2GO and Pathway Keeg service were performed. In this study, the first root transcriptome of yerba matei n response to water stress was generated,contributing to the identification of genes and metabolic pathways involved in drought acclimation to be used in future breeding programs.