Description of two cultivated and two uncultivated new Salinibacter species, one named following the rules of the bacteriological code: Salinibacter grassmerensis sp. nov.; and three named following the rules of the SeqCode: Salinibacter pepae sp. nov., S

VIVER, BARTOMEU; CONRAD, ROTH; LUCIO, MARIANNA; HARIR, MOURAD; URDIAIN, MERCEDES; GAGO, JUAN F. ; SUAREZ-SUAREZ, ANA; BUSTOS CAPARROS, ESTEBAN; SANCHEZ MARTINEZ, RODRIGO; MAYOL, EVA; FEDERICO FASSETTA; PANG, JINFENG; MADALIN GRIDAN, IONUT; VENTER, STEPHANUS; SANTOS, FERNANDO; BAXTER, BONNIE; LLAMES, M.E.; CRISTEA, ARDOJAN; BANCIU, HORIA; HEDLUNG, BRIAN; STOTT, MATHEW; KAMPFER, PETER; AMANN, RUDOLF; SCHMITT KOPPLIN, PHILIPPE; KONSTANTINIDIS, KONSTANTINO; ROSELLÓ-MORA, RAMON

SYSTEMATIC AND APPLIED MICROBIOLOGY

ELSEVIER GMBH

Año: 2023

0723-2020

Current “omics” methods allow thecollection of a large amount of information that helps in describing themicrobial diversity in nature. Here, and as a result of a culturomic approachthat rendered the collection of thousands of isolates from 5 differenthypersaline sites (in Spain, USA and New Zealand), we obtained 21 strains thatrepresent two new Salinibacter species. For these species we propose thenames Salinibacter pepae sp. nov. and Salinibacter grassmerensis sp.nov. (showing average nucleotide identity (ANI) values <95.09% and 87.08%with Sal. ruber M31T, respectively).Metabolomics revealed species-specificdiscriminative profiles. Sal. ruber strains were distinguished by ahigher percentage of polyunsaturated fatty acids and specific N-functionalizedfatty acids; and Sal. altiplanensis was distinguished by anincreased number of glycosylated molecules. Based on sequence characteristicsand inferred phenotype of metagenome-assembled genomes (MAGs), we describe two newmembers of the genus Salinibacter. These species dominated in differentsites and alwaysmcoexisted with Sal. ruber and Sal. pepae. Basedon the MAGs from three Argentinian lakes in the Pampa region of Argentina andthe MAG of the Romanian lake Fără Fund, we describe the species Salinibacterpampae sp. nov. and Salinibacter abyssi sp. nov. respectively (showingANI values 90.94% and 91.48% with Sal. ruber M31T, respectively). Sal.grassmerensis sp. nov. name was formed according to the rules of theInternational Code for Nomenclature of Prokaryotes (ICNP), and Sal. pepae,Sal. pampae sp. nov. and Sal. abyssi sp. nov. , which areproposed under following the rules of the newly published Code of Nomenclatureof Prokaryotes Described from Sequence Data (SeqCode). This work constitutes anexample on how classification under ICNP and SeqCode can coexist, and how theofficial naming a cultivated organism for which the deposit in public repositoriesis difficult finds an intermediate solution.