Ovarian immune and endocrine systems in the polycystic ovary syndrome in pregnant and non-pregnant mice

SANDER V; LUCHETTI CG; GONZALEZ C; DI GIROLAMO G; CHIOCCHIO S; CREMASCH G; MOTTA AB

Embu das Artes, Sao Paulo

Simposio; I Latin-American Symposium on Maternal-Fetal Interaction Placenta-Research ƒt Clinical; 2003

Latin-American Association on Maternal-Fetal Interaction Placenta-Research ƒt Clinical

Regulatory Molecules at Maternal-Fetal Interface P-60 OVARIAN IMMUNE AND ENDOCRINE SYSTEMS IN THE POLYCYSTIC OVARY SYNDROME IN PREGNAT AND NON-PREGNANT MICE Valeria Sander 1, Carolina Griselda Luchetti 1, Claudio Gonzalez  2,Guillermo Di Girolamo  2, Sara Chicchio  3, Graciela  Cremaschi  1, Alicia B Motta 1. Centro de Estudios Farmacológicos y Botánicos, Consejo de Investigaciones Ciuentíficas y Tecnológicas, Argentina. 2 Facultad de Medicina, Depto. Farmacología, 3 Instituto de Neurobiología, Argentina. The purpose of the present work was to study the relationship between the immune and the endocrine systems in the polycystic ovary syndrome (PCOS). We use two polycystic rnouse models induced by dehydroepiandrosterone (DHEA). First; ovarian tissues were obtained from animals treated for 6, 10, 13 consecutive days with DHEA (6 mg/Kg body weight). Histological examinations of ovarian tissue from 13 days revealed cyst formation. However, with only 6 days of DHEA injection, serurn esqradiol (E) levels, the potent inmunomodrilator prostaglandin E (PGE) quantified by radioimmunoassay and the ovarian oxidative status, quantified by malondialdyhyde levels, significantly increased, while serum progesterone (P) were augrnented only at 13 days of treatment. Immunohistochemical assay dernonstrated that ovarian C9)8+ T lymphocyte expression increased but CD4+ T lymphocyte expression diminished after 13 of DHEA treatment. In the second rnurine rnodel, pregnant mice were induced PCOS with DHEA during peri-implantation period. We found dirninished levels of P and an increased E concentration correlated to miscarriage. These results suggest fhat: i) ovarian steroid hormones may regulate ovarian T lymphocyte phenotype expressíon, ii) accumulation of the highest E concentrations induce cyst forrnation, iii) together fhese changes may eromote miscarriage in a murine pregnant PCOS model. Supported by Fundacion Alberto Roemmers