EVALUATION OF THE IMMUGENIC RESPONSE GENERATED BY A NOVEL VACCINE FORMULATION AGAINST NEOSPORA CANINUM INFECTION

BENGOA-LUONI, SA; CORIGLIANO, M; CLEMENTE, M; SANDER, VA

Buenos Aires

Congreso; I LASID Meeting LXIII Argentinean Immunology Society Meeting II French-Argentinean Immunology Meeting; 2015

LATIN AMERICAN SOCIETY FOR IMMUNODEFICIENCIES (LASID), Argentinean Immunology Society (SAI)

Background: Neosporosis is an intracellular coccidian diseasecaused by Neosporacaninum, the main pathogen agent responsible for economic losses in the cattleindustry.There is no effective vaccine to prevent neosporosis.In this study we evaluated the immunogenic response generated by the administration of a novel vaccine formulation including the major surface antigen of N. caninum (NcSAG1) and as adjuvant the 81 KDa heat shock protein (HSP81.2) from Arabidopsis thaliana.Methods: Both recombinant proteins were cloned and expressed in Escherichia coli, and then purified and passed through a polymixin B-agarose column to eliminate endotoxins. BALB/c female mice were intraperitoneally immunized on day 0 and 15 with equimolar quantities of the recombinant proteins:rNcSAG(10 g) alone (rNcSAG1 group) or a mixture of rNcSAG1(10 g) and rAtHsp81.2(30 g) (rNcSAG1+rAtHSP81.2 group) in 200 l of Phosphate Buffer Saline(PBS). Control mice were administered 200 l of PBS (C group).Mice were bled on days 0,15,30,60 and 120 from the tail vein to determine titers of total Immunoglobulin G (IgGt), IgG1 and IgG2a.Results:HighIgGt, IgG1 and IgG2a specific antibody levels directed against recombinant NcSAG1protein were developed byrNcSAG1+ rAtHSP81.2 groupfrom day 30 post immunization, while rNcSAG1 group showed lower titers of IgGt and IgG1on day 30, and IgG2a antibodies were detected only from day 60 post immunization. Conclusions: rNcSAG1combined with the adjuvant rAtHSP81.2 was able to induce an important humoral response in immunized mice, encouraging us to determine the effectiveness of this vaccine formulation in preventing the deleterious effects of congenital neosporosis.