Effects of natural ligands of PPARgamma on lipid metabolism in placental tissues from healthy and diabetic rats.

CAPOBIANCO E; WHITE V; HIGA R; MARTÍNEZ N; JAWERBAUM A

MOLECULAR HUMAN REPRODUCTION.

Oxford University Press

Lugar: Oxford; Año: 2008 vol. 14 p. 491 - 499

1360-9947

The ligand-dependent nuclear receptor peroxisome proliferators-activated receptors (PPARgamma) plays an important role in placental development and function. The aim of this study was to analyse the influence of natural PPARgamma ligands on placental lipid metabolism in diabetic and control rats after midpregnancy, as well as the concentrations of the PPARgamma endogenous agonist 15deoxyDelta(12,14)Prostaglandin J(2) (15dPGJ(2)). In vitro experiments showed that 15dPGJ(2) did not regulate placental concentrations of triglycerides, cholesteryl esters, phospholipids and free fatty acids, but decreased the de novo synthesis of these lipid species. PPAR agonists were administered in vivo through dietary supplementation with either 6% olive oil or 6% safflower oil. These treatments led to increases in placental lipid mass in control tissues and more markedly in diabetic tissues. In addition, they led to reductions in the de novo lipid synthesis both in control and diabetic placental tissues. 15dPGJ(2) concentrations were greatly reduced in the placenta from diabetic rats fed with the standard diet. Both dietary supplementations increased the concentrations of 15dPGJ(2) in placentas from control and diabetic rats. These data indicate that, in the placenta, PPARgamma natural ligands regulate the concentration of their own endogenous ligands. In addition, they increase the placental capacity to accumulate maternal-derived lipids, and reduce the de novo lipid synthesis, thus regulating metabolic pathways that are altered in the placenta from diabetic rats and involved in the lipid transfer to the developing fetus.