Oxidation of BSA photosensitized by Rose Bengal

ARGAÑARAZ, NATALIA MARIEL; ESPECHE TURBAY, M. BEATRIZ; REY, VALENTINA; BORSARELLI CLAUDIO D

Córdoba

Encuentro; XI Encuentro Latinoamericano de Fotoquímica y Fotobiología; 2012

Universidad Nacional de Río Cuarto

Proteins like other biological macromolecules may be targets to the action of reactive oxygen species (ROS). The oxidation of these substrates produces changes in the functionality and/or alterations of the structure causing further changes at physiological level. As a result, the characterization of protein oxidative damage both by 1O2 (singlet oxygen) or by any other ROS, using model systems in vitro is necessary to evaluate the damage occurring in biological media. The aim of this work is to study the oxidation of bovine serum albumin (BSA) by 1O2 generated through photosensitization of Rose Bengal (RB) in homogeneous media. We have focused in the analysis of the photo-oxidation that occurs in the adduct formated using different concentrations of BSA (10M, 50M and 100M) with a fixed concentration of RB (10M), adduct I (1/1), II (1/5) and III (1/10). This was evaluated using various spectroscopic techniques, i.e. UV-Vis absorption and fluorescence, as well as analytical and biochemical techniques (peroxides and carbonyl determination).