Role of PKD1, LIMK1 and BARS in the formation of Golgi outposts in hippocampal neurons

QUASSOLLO GONZALO; GASTALDI LAURA; WOJNACKI JOSE; ALFREDO CACERES

Santa cruz Chile

Congreso; Emerging concepts on neuronal Cytoeskeleton; 2011

p { margin-bottom: 0.21cm; direction: ltr; color: rgb(0, 0, 0); }p.western { font-family: "Times New Roman",serif; font-size: 12pt; }p.cjk { font-family: "Lucida Sans Unicode",sans-serif; font-size: 12pt; }p.ctl { font-family: "Mangal",serif; font-size: 12pt; }Neurons are highly polarized cells that presentstructural and functional asymmetries generated and supported by thecytoskeleton and by the selective, active and constant transport ofmaterials from the Golgi apparatus to their final destinations, whichare fundamental for the maintenance of cellular morphology and itscorrect functioning. In the majority of mammal cells, the Golgiapparatus consists of a stackedarray of cisternae and connecting tubules/vesicleslocated in the perinuclear area close to the microtubule-organizingcenter. In neurons, this arrangement of membrane is partiallydifferent since not only consists of thepericentriolar membrane array found in most cell types but alsoincludes discrete structures dispersed in dendrites termed Golgioutposts. These structures demonstrate theexistence of a new platform in membrane traffic and segregationwithin dendrites. Previousstudies from our laboratory and others have involved LIMK1 and PKD1in the regulation of Golgi dynamics and post Golgi trafficking. Inaddition, recent studies have shown that BARS protein controls thefission/formation of transport carriers. Giventhe importance of these structures, and that their biogenesis andmaintenance are poorly understood; we proposed to study the role ofPKD1, LIMK1 and BARS proteins in the generation and distribution ofGolgi outposts in hippocampal neurons dendrites.In accordance with the observations presented here we can concludethat: (a) LIMK1 regulates the formation of Golgi posts from thesomatic Golgi apparatus, (b) Normal activity of PKD1/BARS also has arole in regulating the number of Golgi posts in dendrites ofdeveloping neurons.