Participation of PKD1, LIMK1 and BARS in the formation of Golgi outpost in hippocampals neurons

QUASSOLLO GONZALO; GASTALDI LAURA; WOJNACKI JOSE; ALFREDO CACERES

Huerta grande Córdoba

Congreso; XXV Reunión anual De La Sociedad Argentina De Investigación En Neurociencias.; 2010

Sociedad Argentina De Investigación En Neurociencias.

p { margin-bottom: 0.21cm; direction: ltr; color: rgb(0, 0, 0); text-align: left; }p.western { font-family: "Times New Roman",serif; font-size: 12pt; }p.cjk { font-family: "Lucida Sans Unicode",sans-serif; font-size: 12pt; }p.ctl { font-family: "Mangal"; font-size: 12pt; }Neurons are cells highly polarized that presentstructural and functional asymmetries generated and supported by thecitoskeleton and by the selective, active and constant transport ofmaterials from the Golgi complex to the final destinations, which arefundamental for the maintenance of cellular morphology and hiscorrect functioning. In the majority of mammal cells, the Golgicomplex consists of a stackedarray of cisternae and connecting tubules/vesiclesof peri nuclear location related to the microtúbules organizingcenter. In neurons, this arrangement of membranes is partiallydifferent since not only consists of a set of peri nuclear cisterns,but also of discreet structures distributed along dendrites,named Golgi outposts. These structures, demonstrates the existence ofa new platform in the membrane traffic and segregation withindendrites. Given the importance of these new structures, we proposedto determine the involvement of PKD1, LIMK1 and BARS proteins in thegeneration and distribution of Golgi outposts in hippocampal neurondendrites.