INVESTIGADORES
PIZZOLITTO Romina Paola
artículos
Título:
Saccharomyces cerevisiae strains from animal environmental with aflatoxin B1 detoxification ability and anti pathogenic bacteria influence in vitro.
Autor/es:
ARMANDO M.R.; DOGI C.; PIZZOLITTO R. P.; ESCOBAR F.; PEIRANO M.S.; SALVANO M A.; SABINI L.; COMBINA M.; DALCERO A. M,; CAVAGLIERI L.R.
Revista:
World Mycotoxin Journal
Editorial:
Wageningen Academic Publishers
Referencias:
Año: 2011 vol. 4 p. 59 - 68
ISSN:
1875-0710
Resumen:
Mycotoxins are metabolites produced by fungi growing on foods or feeds and represent a serious hazard to
humans and animals. Concerns related to the negative health impact of aflatoxins have led to the investigation of
strategies to prevent, eliminate or reduce the presence of these toxins in contaminated products. Saccharomyces
cerevisiae strains are among promising candidates that can be used in animal feed for improving the robustness
of animals in the production environment. The aim of this work was to isolate and select S. cerevisiae strains from
pig environment with aflatoxin B1 (AFB1) binding ability, able to tolerate gastrointestinal conditions and with
some potential beneficial properties to the host. S. cerevisiae strains were isolated from animal feed, faeces and gut
and identified by morphological and molecular techniques. AFB1 binding percentages varied among yeast strains
according to the AFB1 concentration used. The RC016 strain showed the highest adsorption percentage at the three
AFB1 concentrations tested in this work (50, 100 and 500 ng/ml) followed by RC008 strain. All yeast strains were
able to survive under gastrointestinal conditions and to strongly adhere to Vero cells. All S. cerevisiae strains showed
co-aggregation with pathogenic bacteria (Escherichia coli, Enterobacter cloacae and Salmonella enterica sub sp.
enterica). Only RC016 and RC008 strongly inhibited the three pathogens assayed. S. cerevisiae strains RC016 and
RC008 are promising microorganisms for inclusion in animal feed.