High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells

ANCHORDOQUY J MATEO; ANCHORDOQUY J PATRICIO; NIKOLOFF N; PASCUA AM; FURNUS CC

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2017 vol. 24 p. 20041 - 20049

0944-1344

The aim of this study was to investigate the cytotoxicand genotoxic effects of high copper (Cu) concentrationson bovine cumulus cells (CCs) cultured in vitro.We evaluatedthe effect of 0, 120, 240, and 360 μg/dL Cu added to in vitromaturation (IVM) medium on CC viability assessed by thetrypan blue (TB)?fluorescein diacetate (FDA) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) assays, apoptosis, and DNA damage. Differences incell viability assessed by TB?FDAwere not significant amongCC treated with 0, 120, 240, and 360 μg/dL Cu. However,mitochondrial activity assessed by MTT was lower in CCcultured with 120, 240, and 360 μg/dL Cu as compared withthe control (p < 0.01). Percentages of apoptotic cells werehigher when CCs were treated with 120, 240, and 360 μg/dL Cu (p < 0.05) due to higher frequencies of late apoptoticcells (p < 0.05). The frequency of live cells diminished in adose-dependent manner when Cu was added to the culturemedium. Whereas genetic damage index (GDI) increased significantlyin CC cultured in the presence of 240 and 360 μg/dL Cu (p ˂ 0.05), DNA damage increased at all Cuconcentrations tested (p ˂ 0.05). These results indicate thatCu induces cytotoxic and genotoxic effects in bovine CC.