CELLULAR BEHAVIOR OF PROSTATE CANCER IN AN INFLAMMATORY MICROENVIRONMENT

QUINTAR AA; LEIMGRUBER C; MACCIONI M; DOLL A; MALDONADO CA

Río Janeiro

Congreso; 10th International Congress on Cell Biology (ICCB); 2012

It is known that cancer cells can alter their phenotypic features responding to changes in the microenvironment. Our aim is to evaluate the influence of inflammation-modified stromal compartment on prostate cancer cells. To this, we firstly developed a chronic prostatitis model in Copenhagen rats by injecting intraprostatically 107CFU of E.coli, evaluating the gland 28d afterwards (CPr). By immunohistochemistry (IHC), CD3+ and CD8+ infiltrating cells were predominant, with scattered CD4+ and CD11b/c+, in the interstitial infiltrate. Stromal modifications also included hypertrophy and activation of the SMA+ (smooth muscle actin) periacinar layer; whereas both bacterial cultures and IHC were negative for E.coli, indicating its absence in chronic prostatitis. Then, the MatLu prostatic cancer cell line was implanted orthotopically (106cells/rat) into syngenic normal rats (MatLu) or with chronic prostatitis (CPr+MatLu). One month after, the prostate and sex accessory glands were evaluated by electron microscopy and proliferating tumoral cells determined by IHC of BrdU-incorporating cells. Tumoral weight was slightly lower in CPr+MatLu, with a significant decrease in the mitotic index, BrdU+ cells (p