Testosterone modulates prostate smooth muscle cell response to bacterial LPS

LEIMGRUBER C; QUINTAR AA; MALDONADO CA

Río Janeiro

Congreso; 10th International Congress on Cell Biology (ICCB); 2012

Prostate smooth muscle cells(pSMCs) play a key role in prostate physiology regulated by Testosterone. We previously demonstrated that LPS induces dedifferentiation and proinflammatory response of pSMCs. Our aim was to elucidate a possible modulation of pSMC response to LPS by Testosterone. Rat pSMCs were treated in vitro with LPS (1 or 10 µg/ml) for 24 or 48h with or without Testosterone(10-7M). Ultraestructural examination showed well-developed proteinopoietic organelles and loss of the contractile apparatus after LPS. Meanwhile, pSMCs conserved a more-differentiated phenotype with Testosterone. Furthermore, LPS challenge decreased ACTA2 filaments and calponin expression (p