An inflammatory background influences the growth of prostate cancer.

QUINTAR A.A.; LEIMGRUBER C.; MALDONADO C.A.; MACCIONI M

Viña del Mar

Congreso; 9th Latin American Congress of Immunity.; 2009

Sociedad Argentina de Inmunología, Sociedad Chilena de Inmunología, Latin American Association of Immunology

An inflammatory background influences the growth of prostate cancer Amado A Quintar1, Carolina Leimgruber1, Cristina A Maldonado1, Mariana Maccioni2 1 Centro de Microscopía Electrónica. Facultad de Cs. Médicas. Universidad Nacional de Córdoba, Argentina 2 CIBICI-CONICET, Dpto Bioquímica Clínica. Facultad de Cs. Químicas. Universidad Nacional de Córdoba, Argentina There is much evidence supporting that stromal environment dictates the tumoral progression. Our aim is to analyze the impact of inflammation-modified stromal microenvironment on prostate cancer growth. For this purpose, we firstly developed a chronic prostatitis model in Copenhagen rats by injecting intraprostatically 107 CFU of E.coli and evaluating the gland 28d afterwards (CPr). By immunohistochemistry (IHC), CD3+ and CD8+ cells were predominant in the interstitial infiltrate, with scattered CD4+ and CD11 b/c+. Stromal modifications also included hypertrophy and activation of the aSMA+ (smooth muscle actin) periacinar layer; whereas both bacterial cultures and IHC were negative for E.coli, indicating its absence in chronic prostatitis. Subsequently, the MatLu prostatic cancer cell line was orthotopically implanted (106 cells/rat) into syngenic normal rats (MatLu) or with chronic prostatitis (CPr+MatLu). Twenty days after, sex accessory glands were evaluated, being proliferating tumoral cells determined by IHC of BrdUincorporating cells. Tumoral weight was slightly lower in CPr+MatLu, with a significant decrease in the mitotic index and BrdU+ cells (p