"Biotest of acute toxicity using biological system of different structural complexity".

BISOGNO,F; MASCOTTI.M; KURINA SANZ,M; ENRIZ,D; GIANNINI,F

Villa Giardino Córdoba Argentina

Congreso; XV Bienial Scientific Meeting Cordoba Society for Biology; 2005

Cordoba Society for Biology

Biotests of acute toxicity using biological systems of different structural complexity Bisogno F. R.#, Mascotti, M. L*, Kurina M. #, Eiriz, R. D* y Giannini, F.* #INTEQUI-CONICET. Laboratorio de Química Orgánica. *Cátedra de Química General. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Introduction: Biotests using experimental models of different structural complexity, may be used to determinate the potential bioactivity of new compounds, evaluate the acute toxicity of drugs and even to measure toxicity and possible ambient impact of effluents.  Methodology: The design and development of the acute toxicity tests is based on an experimental technique adapted by our group of research, from the tests used by U.S. Fish and Wildlife Service. Columbia National Fisheries Research Laboratory (Waynon W. 1980) to evaluate acute toxicity of diverse chemical compounds. As experimental models were used fish like Poecilia reticulata, mollusks like Lymnaea sp. and amphibious as Xenopus laevis and Buffo arenarum (larva). In order to demonstrate the potentially of those biotests and try to validate their correlation wit the bioactivity of mentioned drugs, molecules of diverse nature and origin were chosen. Results and discussion: The highest levels of toxicity were the corresponding to compounds reported as bioactive. Likewise the drugs that presented little or none toxicity were submit to the evaluation of other bioactivities such as antifungic activity, obtaining results correlated to the ones of toxicity. This proves that the tests here exhibited are good indicators of the bioactivity of the compounds.