Candida albicans infection in Central Nervous System (CNS): histopathological finding and glial reactivity

PERALTA RAMOS JM; FIGUEREDO CM; CEJAS H; RENNA M.S; SOTOMAYOR CE

Congreso; II Reunión Conjunta de la Sociedad Argentina de Neurociencias y el Taller Argentino de Neurociencias: XXV Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN) y XII Taller Argentino de Neurociencias (TAN). Córdoba, Octubre de 201; 2010

Candida infection in CNS is a serious form of bloodstream infection with 50 % of mortality. With the aim to explore the ethiopathogenic mechanisms in this mycosis, we developed an in vitro model in C57BL/6 mouse. The animals were injected with 5.105, 1.106, or 2,5.106 viable C. albicans and at 12, 24, 48 and 72h the brain was removed. CNS yeast infection was confirmed by CFU and fungal morphotypes were visualized in the lesions (PAS). The local inflammatory reaction was characterized by meningitis and microabscesses distrubuted in the brain parenchymal (HE). The immunostaining with anti-GFAP(astrocytes As marker), revealed the presence of reactive astrogliosis(IF). Interestingly we detected neuronal cell death associated to the infection (FJB/Ag-stain). We also developed as In vitro model to explored the immune reactivity of glial cells against C.albicans. FACS analysis of primary cultures revealed the follow composition: As enriched cultured >96%GFAP+ cells vs 80% vs M