Candida albicans and C6 astrogial cell interaction. Effect on L-arginine pathway”

FIGUEREDO CM; RENNA M.S; PARAJE M.G; CORREA S.G; SOTOMAYOR C.E

Córdoba

Congreso; VII Latin American Congress of Immunology.; 2005

More than the 50% of patient who die form systemic candidiasis show fungal invasion in the central nervous system(CNS). Astrocytes are the most numerous cells type in the brain and can exhibit immunocompetent function in response to injury, such as cytokine release and activation of inducible isoform of nitric oxide synthase(iNOS). This cell has important storage of Arginine, a only substrate of NOS. We evaluate the interaction between rat astroglial line, C6 and C.albicans using FITC-labeled-fungus for microscopy studies and evaluating the ability of C.albicans to trigger the L-arginine pathway. Astroglial cells were co-cultured with viable C.albicans (10:1 effector-target ratio/ 48h), or with LPS and PMA. Specific enzyme inhibitor or different sugars also were employed. C.albicans triggered the classic pathway of L-arginine, showing a clear expression of iNOS (western blot) and significant production of NO(p vs basal and LPS < 0,01); the expression of the enzyme was blocked by Aminoguanidine. Interestingly, C.albicans also trigger in the astroglial cells the alternative metabolic pathway of arginine, activating L-arginase enzyme to produce urea and ornithine. The arginase activity was notably increased after C.albicans incubation, five fold increase compared with the control(p<0.01); mannose 30mM partially blocked the activation. C.albicans can interact with astroglial cells and activate both metabolic pathways. This phenomenon could be important in the immunopathogenesis of CNS candidiasis. the astroglial cells the alternative metabolic pathway of arginine, activating L-arginase enzyme to produce urea and ornithine. The arginase activity was notably increased after C.albicans incubation, five fold increase compared with the control(p<0.01); mannose 30mM partially blocked the activation. C.albicans can interact with astroglial cells and activate both metabolic pathways. This phenomenon could be important in the immunopathogenesis of CNS candidiasis. the astroglial cells the alternative metabolic pathway of arginine, activating L-arginase enzyme to produce urea and ornithine. The arginase activity was notably increased after C.albicans incubation, five fold increase compared with the control(p<0.01); mannose 30mM partially blocked the activation. C.albicans can interact with astroglial cells and activate both metabolic pathways. This phenomenon could be important in the immunopathogenesis of CNS candidiasis. the astroglial cells the alternative metabolic pathway of arginine, activating L-arginase enzyme to produce urea and ornithine. The arginase activity was notably increased after C.albicans incubation, five fold increase compared with the control(p<0.01); mannose 30mM partially blocked the activation. C.albicans can interact with astroglial cells and activate both metabolic pathways. This phenomenon could be important in the immunopathogenesis of CNS candidiasis. C.albicans using FITC-labeled-fungus for microscopy studies and evaluating the ability of C.albicans to trigger the L-arginine pathway. Astroglial cells were co-cultured with viable C.albicans (10:1 effector-target ratio/ 48h), or with LPS and PMA. Specific enzyme inhibitor or different sugars also were employed. C.albicans triggered the classic pathway of L-arginine, showing a clear expression of iNOS (western blot) and significant production of NO(p vs basal and LPS < 0,01); the expression of the enzyme was blocked by Aminoguanidine. Interestingly, C.albicans also trigger in the astroglial cells the alternative metabolic pathway of arginine, activating L-arginase enzyme to produce urea and ornithine. The arginase activity was notably increased after C.albicans incubation, five fold increase compared with the control(p<0.01); mannose 30mM partially blocked the activation. C.albicans can interact with astroglial cells and activate both metabolic pathways. This phenomenon could be important in the immunopathogenesis of CNS candidiasis. C.albicans incubation, five fold increase compared with the control(p<0.01); mannose 30mM partially blocked the activation. C.albicans can interact with astroglial cells and activate both metabolic pathways. This phenomenon could be important in the immunopathogenesis of CNS candidiasis.