Assessment of HSP70 and catalase in Brachidontes rodriguezii (d'Orbigny, 1842) a mussel from the Argentinean coast.

MATOS B. ; PINONI S; MARCOVAL A; DÍAZ-JARAMILLO M; DINIZ, M.S.

Congreso; 3rd International Congress of CiiEM-2018; 2018

Introduction: Intertidal ecosystems are complex environments of great importance for the ecological balance of coastal zones and are vulnerable areas to effects caused by natural stressors of global relevance such as temperature. Mussels, are often exposed to drastic variations of temperature and can show intracellular mechanisms of compensation that allow them to counteract exposure in the short and medium term at high temperatures. Heat shock proteins are chaperones that allow thermal stability of proteins against abrupt changes in temperature and catalase is an antioxidant enzyme that aid cells to fight against oxidative stresscaused by diverse environmental stressors [1,2].Materials and Methods: Mussel samples were homogenized in PBS buffer (phosphate saline solution, pH=7.4). centrifuged (10,000xg for 15 min at 4ºC). Afterwards, the supernatants were transferred to 1.5 ml microtubes and stored at -80ºC until analysis. The HSP70 levels were determined in individuals from both sexes, exposed at different temperatures (20ºC, 24ºC, 26ºC and 28ºC) for 7 and 14 days. Organisms sampled at T0 were also analysed. The HSP70 levels were determined by ELISA (Enzyme Linked Immunosorbent Assay) as described by Madeira et al. [2] and catalase following the method described in Diniz et al. [3].Results and Discussion: The preliminary results show an absence of significant differences between sexes of HSP70 levels at T0, 20 ° C and 24 ° C. However, at 26°C and 28°C, a differentiated response was observed in organisms exposed at high temperatures, where the females had higher levels of HSP70 in the short term (7d) compared to male individuals (p