Identificación de genes xilanasa a partir de una cepa de Pseudobutyrivibrio xylanivorans

GRILLI, D.; KOPECNY, J; MRAZEK, J; CERÓN, M.; SCHNITTGER, L.; CRAVERO, S; SOSA ESCUDERO, M; ARENAS, GN.

San Luis

Jornada; XXX Reunión Anual de la Sociedad de Biología de Cuyo.; 2012

Sociedad de Biología de Cuyo

Genes encoding glycosyl hydrolase family 11 (GH 11) xylanases and multiple xylanases have been identified from Pseudobutyrivibrio xylanivorans. However, relatively little is known about the diversity and distribution of the GH 10 xylanase genes in strains of P. xylanivorans. The xylanase and associated activities of P. xylanivorans have been characterized in greatest detail from the type strain, Mz5. In present study, we evaluated degradation and utilization of xylan by wild-type strain of P. xylanivorans isolated from rumen of Creole goats. P. xylanivorans 2 cultures were able to utilize up to 53% of the total pentose present in birchwood xylan (BWX), after 12 h, but could utilize up to 62% of a water-soluble fraction prepared from BWX. In addition, we propose to identify GH 10 xylanase genes in strain 2 and Mz5 of P. xylanivorans. This is the first report of successful PCR amplification using primers for GH 10 xylanase-encoding genes in P. xylanivorans. Strain 2 contained xylanase gene clearly related to xynA family of Butyrivibrio-related strains. The xylanase gene sequence of P. xylanivorans Mz5 was designated to the xynB family. These genes were phylogenetically distanced, derived from a common ancestor but evolved independently, adapting to particular environmental conditions for two different host species.