Pseudobutyrivibrio ruminis AISLADO DEL RUMEN DE CABRAS BIOTIPO CRIOLLO. IDENTIFICACIÓN GENÉTICA Y BIOQUÍMICA.

GRILLI, D.; CERÓN, M. ; SCHNITTGER, L.; PAEZ, S.; EGEA, V. ; COBOS, E.; ALLEGRETTI, L.; ARENAS, GN.

San Juan

Jornada; II Reunión Conjunta de Sociedades de Biología de la República Argentina.; 2011

Sociedades de Biología de la República Argentina.

In the region of Lavalle, Mendoza; goats compose their diet with a high proportion of shrub species, which constitute a major supply of fiber. The high efficiency in the utilization of the fiber may be due, among other factors, to characteristics of ruminal fibrolytic bacteria. Therefore, the study of these bacteria assumes great importance in goat production systems in our country. The aim of this study was to identify and characterize through genetic and biochemical tests, the cellulolytics bacteria isolated from rumen of Creole goats. Sequences of the gene (DNA) encoding subunit 16S ribosomal RNA (16S rDNA) are a powerful tool for taxonomic classification of rumen bacteria and to demonstrate the vast microbial diversity present in the rumen of these animals. We isolated 13 strains of strictly anaerobic bacteria from rumen of Creole goats grazing on rangelands of NE Mendoza. One of them was genetically identified using 16S rDNA sequencing, morphology and physiological characteristics and fermentation end products. The results support the designation of this strain as belonging to the genus Pseudobutyribivibrio ruminis, which is closely related to Butyrivibrio fibrisolvens. These biotypes are predominant in rumen of animals adapted to rigorous conditions feeding and diets of poor nutritional quality. It is the first report the isolation and identification of a bacterial strain from rumen contents of Creole goats.