Natural 1,25(OH)2D3-glycosides compared to synthetic1,25(OH)2D3 on skeletal muscle cells differentiation

GILI, V; DE GENARO, P; BACHMANN, H; BOLAND, R; RUSSO DE BOLAND, ANA

Congreso; XXXII Reunión Anual de la Asociación Argentina de Osteología y Metabolismo Mineral; 2016

The presence of glycoside derivatives of 1α,25(OH)2D3 endows plants to gradualrelease of the free bioactive form of 1α,25(OH)2D3 from its glycoconjugates by endogenous animal tissue glycosidases. This results in increased half-life of the hormone in blood when purified plant fractions are administered for therapeutic purposes. The objective of this work was to evaluate the role of 1α,25(OH)2D3-glycosides from Solanum glaucophyllum leaf extract compared with synthetic 1α,25(OH)2D3 on myogenic differentiation in C2C12 myoblasts. Cellular mass increased with myogenesis progression and was reflected in the morphology of cultured muscle fibers. Western blot analysis showed that 1α,25(OH)2D3-glycoside induced increases in the specific muscle proteins tropomyosin (20%, p b 0.05) and myogenin (80%, p b 0.05) expression, at day 2 of differentiation. Myosin heavy chain (MHC), a mature muscle cells marker, increased 50% at day 4. Treatments did not affectmyogenin and MHC cell localization. Likewise the synthetic hormone, 1α,25(OH)2D3-glycoside (1 nM) increased by 60% (p b 0.05) the activity of the enzyme creatine kinase (CK) at the second day after induction of differentiation. In all cases, the effect of 10 nM 1α,25(OH)2D3-glycoside was more attenuate. 1α,25(OH)2D3-glycoside as well as synthetic 1α,25(OH)2D3, augmented the index fusion of cultured muscle fibers as was observed by fluorescence microscopy. These results demonstrated that plant 1α,25(OH)2D3-glycosides exhibit at least equal or greater effects on early myoblast differentiation as the animal hormone, suggesting that the plant glycoside could be an effective cheaper substitute for synthetic 1α,25(OH)2D3 to promote muscle growth