Isolation and Characterization of vB_MsmS_Celfi, a new M. tuberculosis bacteriophage

PAYASLIAN, FLORENCIA 1,2; GRADASCHI, VICTORIA; RONDÓN SALAZAR, LILIANA; DIETERLE, MARIA EUGENIA ; URDÁNIZ, ESTEFANÍA; DI PAOLA, MATIAS; PEÑA CÁRCAMO, JOSÉ; ZON, FABIO; SOSA EZEQUIEL; FERNANDEZ DO PORTO, DARÍO ; MATTHEW DUNNE; PAULINE GOELLER; JOCHEN KLUMPP; RAYA RAUL; REYES, ALEJANDRO; PIURI, MARIANA

phage: therapy applications and research

Mary Ann Liebert

Año: 2020

2641-6530

Introduction: Because of the clinical relevance of Mycobacteria, and from a therapeuticperspective, there is an increasing interest to study phages that infect bacteria belonging to thisgenus.Materials and Methods: A phage was isolated from a soil sample, using M. smegmatis as host.Its characterization included, sequencing, annotation and analysis of the genome, host rangedetermination and electron microscopy imaging.Results: Mycobacterium phage vB_MsmS_Celfi is a temperate phage able to infect M.tuberculosis with high efficiency. From electron microscopy images Celfi belongs tothe Siphoviridae family. Genome analysis classified phage Celfi into cluster L, subcluster L2 of Actinobacteriophage clusters. Mycobacterium phage Celfi exhibits a Lysin B distant to thosepresent in other members of the subcluster and other mycobacteriophages.Conclusions: The discovery of new phages that infect M. tuberculosis could contribute to thedevelopment of novel tools for detection systems and future treatment of the disease.