A PRO12ALA POLYMORPHISM IN THE PPARgamma2 GENE ASSOCIATED WITH LIPID PROFILE IN T2DM

OLMOS NICOTRA MF; SIEWERT S; FILIPUZZI S; GONZÁLEZ I; OJEDA MS

CUIDAD DE LA PUNTA

Congreso; XXVII REUNIÓN CIENTÍFICA ANUAL SOCIEDAD DE BIOLOGÍA DE CUYO; 2009

SOCIEDAD DE BIOLOGÍA DE CUYO

Peroxisome proliferator-activated receptor gamma 2 (PPAR gamma2)controls adipocyte differentiation and regulates lips and glucose homeostasis. The substitution of proline to alanine at codon 12 of the PPARgamma2 (PRO12ALA polymorphism) is most widely studied. The aim of this study was to estimate the allele frequency of the Pro12Ala polymorphism of the PPARgamma2 gene and investigate the association between this polymorphism and the type 2 diabetes (T2DM). Our sample comprised diabetic patients and healthy controls. Biochemical measurements were: HDLc, LDLc, total colestrol (TC), triglycerides (TG) and Pro12Ala PPAR gamma2 polymorphism were also determined using Tetra-primer ARMS-PCR method. The frecuency of the genotype Pro/Ala was significant different among diabetic patients and the control group, 13.3% vs. 25% respectively. All genotypes frecuencies were in Hardy Weinberg Equilibrium. Levels of serum TG in diabetic patients were significantly higher in the Pro/Pro genotype than in the Pro/Ala and Ala/Ala genotypes (p