Meganuclease I-SceI Enhances Stable Transgene Integration in Cultured Bovine Fetal Fibroblasts

ORTEGA NM; BENITEZ SB; BARRIONUEVO BE; OLMOS NICOTRA MF; ALESSIO AP; FILI AE; FORCATO DO; STICE SL; BOSCH P.

Hannover

Congreso; 39th Annual Conference of the IETS; 2013

Australian Academy of Science

Production of genetically modified large animals through somatic cell nuclear transfer (SCNT) requires genetic manipulation of cultured cells, which are subsequently used as nuclear donor to generate a transgenic animal. Stable transgene integration into donor cell genome is an inefficient process that involves integration of transgenes in randomly occurring DNA double-strand breaks. Therefore, our objective was to evaluate transient and stable transfection in cultured bovine fetal fibroblasts (BFF) using a transgenic strategy based on the simultaneous presence of a meganuclease (I-SceI) and a transgene flanked by restriction sites for I-SceI. BFF (2.63 x 10 4 cells/cm 2 in 24-well plates) were cotransfected with a plasmid vector (pBSII-I-SceI-ZsGreen1-Neo) carrying expression cassettes for ZsGreen1 (fluorescent protein) and neomycin resistance flanked by restriction sites for I-SceI along with an expression plasmid for I-SceI (pCBASce). As controls, BFF were cotransfected with pBSII-I-SceI-ZsGreen1-Neo plus a plasmid that lacks I-SceI expression cassette (pCBA). Lipofectamine 2000 (Invitrogen) was used as transfection reagent as per manufacturer´s instructions. Two different relationships of vector pBSII-I-SceI-ZsGreen1-Neo to pCBASce or pCBA (control) were tested: 1:1 and 1:3 (total amount of plasmid DNA per well was 500 ng). Transient transfection was evaluated by flow citometry and reported as percentage of green fluorescent cells 72 h post-cotransfection. Stable integration of transgene sequences was assessed 21 days after cotransfection by determining the number of fluorescent cell colonies (FCC) that developed in selective media (DMEM + 250 μg/mL of G418). Data were analyzed by ANOVA and Tukey test and expressed as mean ± SEM. Flow cytometric analysis at 72 h post-transfection showed no statistical differences between the proportions of fluorescent cells in cultures cotransfected with pCBASce compared with those transfected with the control plasmid. The number of FCC developed from cultures cotransfected with pBSII-I-SceI-ZsGreen1-Neo plus pCBASce at ratio 1:1 was 6.4-fold higher compared to those observed in the control group at the same ratio (8.00±2.16 vs 1.25±0.62 colonies; p=0.02). However, there was no difference in the number of FCC formed at plasmid ratio 1:3 between the treatment (3.75±1.03 colonies) and the control (2.70±1.35 colonies; p>0.05). Several transgenic BFF cell lines were generated by subculturing individual colonies. PCR and Southern blotting confirmed that antibiotic resistant and phenotypically positive colonies had integrated the ZsGreen1 transgene. Western blot analysis using an anti-HA antibody revealed a band of the expected size (30 kDa) in cells transfected with pCBASce. We conclude that I-SceI transgenesis significantly increases the functional integration of plasmid DNA into the bovine fibroblast genome as it has been reported in embryos of other vertebrates, up to now by unknown mechanisms. This transgenic strategy should facilitate stable transfection of bovine fibroblasts to generate genetically modified animals though SCNT.