Transcriptional pofile changes in grapevine (Vitis vinifera L.) cv. Malbec induced by UV-B radiation

LIJAVETZKY, D; PONTIN, M; BOTTINI R; PICCOLI, P; MARTINEZ-ZAPATER, JM

Adelaide

Simposio; Eighth International Symposium on Grapevine Physiology and Biotechnology; 2008

Australian Society of Viticulture and Oenology

Background and AimsUltraviolet-B radiation (UV-B) is a natural component of the solar radiation that affects plant growth and development. In Mendoza (Argentina), vineyards with top reputation for premium wines are those located at altitudes of 1500 m, where UV-B reaches high values, with consequences on berry growth and quality. An analysis of the transcriptional profile changes produced by different UV-B treatments on grapevine plants was undertaken.Methods and ResultsGrapevine cv. Malbec plants were grown in vitro 45 d under PAR (100 μmol m-2 s-1) and then subjected for one day to an UV-B dose similar to the radiation registered at 1500 m of altitude but with two different distributions. The treatments were: a) 16 h PAR without UV-B (control); b) 16 h PAR + 16 h UV-B (7,2 μW cm-2); c) 16 h PAR + 4 h UV-B (28,5 μW cm-2). Total RNA was isolated from apical leaves and used to hybridize grapevine custom Affymetrix GeneChips (developed by the GrapeGen project) containing 23000 probe-sets. For the “4 h” treatment, a total of 4029 genes were differentially expressed (p