Evolution of LysM-RLK family in wild and cultivated peanut species

RODRIGUEZ-MELO, JOHAN STIBEN; BARBOSA, MARÍA CAROLINA; ARIEL, FEDERICO; WANG, JEANPIN; FABRA, ADRIANA; TONELLI, MARÍA LAURA; IBAÑEZ, FERNANDO

Congreso; XXXIII Argentinian Meeting of Plant Physiology; 2021

Evolution of LysM-RLK family in wild and cultivated peanut speciesJ.S Rodríguez, M.C. Barbosa, F. Ariel, A. Fabra, M.L. Tonelli, F. IbañezLysM-RLKs are transmembrane proteins containing three extracellular LysM domains, a transmembrane region and an intracellular kinase domain. These proteins are exclusively found in plants and are crucial for differentiation among structurally similar signaling microbial molecules or patterns. In legumes, LysM-RLK perception of rhizobial Nod factors triggers a signaling pathway related with the onset of symbiosis development. On the other hand, activation of LysM-RLKs after binding of chitin-derived short-chitooligosaccharides initiates defense responses. In this work, we identified the members of LysM-RLK gene family in cultivated (A. hypogaea L.) and wild (A. duranensis and A. ipaensis) peanut genomes and reconstructed the evolutionary history of the family. Within this phylogenetic framework, we reinterpreted the functional data reported for peanut LysM-RLKs. In addition, potential roles of two identified proteins during Nod factors and chitosan inoculation were assessed by gene expression analyses and protein modelling. Results indicated that peanut LysM-RLK is a highly diverse family. Digital expression analyses indicated that some A. hypogaea LysM-RLK receptors were up regulated during early and late stages of symbiosis. Also, expression profiles and 3D analysis of selected receptors revealed particularities possibly related with their capacity to recognize Nod factors and/or chitosan.Analyses of LysM-RLK in peanut, a non-model legume, can contribute to gain insight into perception of microbial signals during the legume-microbe interactions and to the understanding of the evolutionary history of this gene family within the Fabaceae.