INVESTIGADORES
TONELLI Maria Laura
artículos
Título:
Biocontrol Bacteria Communities Associated with Diseased and Healthy Peanut (Arachis hypogaea L.) plants
Autor/es:
LILIANA LUDUEÑA; TANIA TAURIAN; M. LAURA TONELLI; JORGE G. ANGELINI; FERNANDO IBÁÑEZ; M. SOLEDAD ANZUAY; LUCIO VALETTI; ADRIANA FABRA
Revista:
EUROPEAN JOURNAL OF SOIL BIOLOGY
Editorial:
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Referencias:
Lugar: Paris; Año: 2012 vol. 53 p. 48 - 55
ISSN:
1164-5563
Resumen:
In the present study, the isolation and characterization of endophytic and epiphytic biocontrol bacteria
from peanut plants affected with white mold and root rot are described. Two hundred and sixty three
isolates were selected from diseased plants and biocontrol traits analyzed were antibiosis against Sclerotinia
minor and Fusarium solani, siderophore production and similarities in carbon source utilization
between bacteria and fungi. Diversity and sequence analysis of potential biocontrol bacteria were
developed. It was observed that 34% of the 263 isolates were able to inhibit the growth of at least one of
the two fungi tested and 47% showed siderophore production in CAS medium. Analysis of nutritional
similarity index demonstrated that both fungi were capable to growth in all carbon compounds assayed
and that 20% of bacteria showed NOI values equal or above 0.9. Genetic diversity analysis by BOXePCR
indicated that culturable epiphytic and endophytic bacteria associated with peanut are highly diverse.
The 16S rDNA sequences of the most remarkable isolates, indicated as potential biocontrol agents
according to all the screenings performed and belonging to different BOX profiles showed that they were
99% identical to Bacillus amyloliquefaciens.
Comparisons analysis was performed with results previously obtained from a similar study done in the
bacterial community isolated from healthy peanut plants from the same producing area. Results obtained
suggest that presence of fungal pathogens in peanut ecosystem would be acting as a selective factor in
the peanut plant associated bacterial communities. It was concluded from this study that peanut soils in
Cordoba harbor bacteria with major biocontrol properties which represent a potential source of new
strains that could be used as biological inoculants in agriculture.