DIFFERENT RESPONSES IN ANXIOGENIC-LIKE BEHAVIOR AND MOTOR COORDINATION IN CB1+/- AND CB1-/- MICE

SORIANO DELIA; BRUSCO HA; CALTANA LAURA

Carlos Paz

Congreso; XXXIV Reunión Anual SAN; 2019

Sociedad Argentina de Investigación en Neurociencias

Given that theCB1 receptor (CB1) has been associatedto mood and behavior, the aim of thisstudy was to evaluate the effects of CB1deficiency in behavioral paradigms ofanxiety and depression. Male CB1 knockout mice (CB1-/-), heterozygous CB1+/-andCB1+/+ were tested in behavioral paradigms. Open field (OF), elevated plusmaze(EPM), light-dark box (LDB) and rotarod tests were carried out toevaluateanxiety-like behavior and motor coordination. Novelty-suppressedfeeding (NSF),sucrose splash and forced swimming test (FST) were performed toevaluatedepression-like behavior. In EPM and LDB, the number of entries andtime spent in the aversive area waslower in CB1-/- but not in CB1+/-. In OF,EPM and LDB, explorative parameterswere decreased only in CB1-/-. In therotarod test, CB1-/- did not improvemotor performance after training whileCB1+/- improved it after repetitivetraining. NSF showed no differences inintake latency but a reduction in CB1-/- foodintake. The sucrose splash testrevealed a reduction in latency to groom andtime of grooming in CB1-/-, and theFST showed longer immobilization during thelast period of time. In conclusion,CB1-/- exhibited deficits in motorcoordination, anxious and depressivebehavior. However, CB1+/- showedalterations in motor coordination but not inanxiety-like behavior. These results could explain the role of CB1 inbehavioralparameters, which may correlate with CB1 polymorphism observed indepressivepatients.