Antibacterial alginate/nano-hydroxyapatite composites for bone tissue engineering: assessment of their bioactivity, biocompatibility, and antibacterial activity.

BENEDINI LUCIANO; LAIUPPA, JUAN A.; SANTILLÁN, GRACIELA E.; BALDINI, MÓNICA; MESSINA PAULA V.

MATERIALS SCIENCE & ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2020 vol. 115

0928-4931

Bone substitutematerials based on bioceramics and polymers have evolved shifting from apassive role where they are merely accepted by the body; to an active role,where they respond to particular environmental conditions or to different typesof cues generating suitable integration (osseointegration for this case) insidethe host tissue. In this work, two types of composite materials based on abioceramic (synthetic nano-hydroxyapatite, HA) and a biopolymer (sodium alginate,ALG) have been designed and assessed for promoting the bone regeneration. Thesematerials were loaded with ciprofloxacin (CIP) for obtaining, not only a suitablematerial for a filling but with antibacterial properties. Therefore, their mainfeatures were studied through Fourier Transformed-Infrared spectroscopy(FT-IR), x-Ray Diffraction (xRD) and transmission electron microscopy (TEM).Ultraviolet-Visible (UV?Vis) spectroscopy was used for obtaining the releasedconcentrations of CIP and Zeta-potential (ζ-potential) was used forcharacterizing the adsorption of CIP onto nanoparticles. The release profile ofthis drug has been fit with the Ritger-Peppas model, used for studying therelease kinetics of hydrogel-based systems. The bioactivity of these compositeswas also evaluated after 30 days of incubation in a simulated body fluidsolution (SBF). Then, the assessment of antibacterial capability against thethree main strains cause osteomyelitis was performed. Finally, the cellviability study and the cellular morphology assay were also carried out. Theselast assays have shown encouraging results and, gathered with their otherproperties, such as their bioactivity and antibacterial properties; they couldlead to propose these materials as new bone filler antibiotic devices.