INVESTIGADORES
TONDO Maria Laura
congresos y reuniones científicas
Título:
Identification of o-linked protein glycosylation system in Ralstonia solanacearum
Autor/es:
ELHENAWY W.; SCOTT N.E.; TONDO, M.L.; ORELLANO, E.G.; FELDMAN M.F.
Lugar:
Canmore
Reunión:
Simposio; 10th Annual National Carbohydrate Symposium of the Alberta Glycomics Centre; 2014
Resumen:
Ralstonia solanacearum is a prominent plant
pathogen known to infect a wide range of crops. R. solanacearum causes
wilt disease leading to economical losses in the agricultural sector worldwide.
Our in-silico analysis suggested the presence of a putative protein
O-glycosylation system in R. solanacearum. The O-glycosylation machinery
was shown to be important for virulence in different bacteria. While some
bacteria utilize cytoplasmic glycosyl transferases to sequentially decorate
their proteins with sugars, others rely on an O-oligosaccharyl
transferase to glycosylate their proteins via en-bloc transfer of
glycans in the periplasm. In this work, we used mass spectrometry to identify
the glycoproteins of R.solanacearum. Our MS analysis revealed the
presence of many glycoproteins with different glycans linked to their serine or
threonine residues via O-glycosylation. Among these glycoproteins is the type
IV pilin known to be important in biofilm formation and twitching motility.
Furthermore, we identified the O-oligosaccharyl transferase (O-Otase)
in R. solanacearum and
demonstrated its activity in vivo. Knocking down the Ralstonia O-Otase
resulted in the loss of protein O-glycosylation as well as a significant
decrease in virulence in tomato plant model. Our data suggests an important
role of O-glycosylation in R. solanacearum which can be a novel target
for new treatments.