On-line biomass monitoring in bench-scale stirred bioreactors using parts of a liquid chromatography system

FORCATO DIEGO OSCAR; PÉCORA ROLANDO PASCUAL; KIVATINITZ SILVIA CLARA

BIOTECHNOLOGY LETTERS

SPRINGER

Año: 2002 vol. 24 p. 1999 - 2003

0141-5492

A cell density monitor, comprising parts of a liquid chromatography system, was adapted for on-line real-time meaA cell density monitor, comprising parts of a liquid chromatography system, was adapted for on-line real-time measurement of bacterial cell density up to 75 g l–1. The device dilutes the sample containing cells and measures the absorbancy at 280 nm. This detector gives an excellent correlation with off-line measurements, good reproducibility, a low limit of detection (3.5 mg l–1), and becomes a low cost alternative for fermentation monitoring in small stirred bioreactors.surement of bacterial cell density up to 75 g l–1. The device dilutes the sample containing cells and measures the absorbancy at 280 nm. This detector gives an excellent correlation with off-line measurements, good reproducibility, a low limit of detection (3.5 mg l–1), and becomes a low cost alternative for fermentation monitoring in small stirred bioreactors.