Spleen alterations and increased brain CD4+ lymphocytes after pilocarpine-induced Status Epilepticus (SE)

PAULA SARCHI; ALICIA ROSSI; MIRIANA MARIUSSI; JERONIMO AUZMENDI; A. JAVIER RAMOS

C{ordoba

Congreso; XXXIII Congreso Anual SAN; 2018

SAN

Epilepsy is one of the most frequent neurological diseases worldwide. A high percentage ofpatients with temporal lobe epilepsy (TLE) refer an initial precipitating event, such as febrileseizures, during childhood, followed by a silent latency period (LP), until the onset of the chronicseizures phase. In an experimental model of TLE, we have previously shown thatneurodegeneration, reactive gliosis and macrophages brain infiltration occur during the LP andthat early interventions limiting glial and immune activation during the LP increase epilepticthreshold during the chronic phase (Rossi et al., 2013; 2017). We here studied the immune cellsparticipation in the LP that follows pilocarpine-induced SE. Male Wistar rats were treated withlithium-pilocarpine (127 mg/kg /30 mg/kg) developing SE that were limited to 20 min by 20 mg/kgi.p. diazepam. After 3DPSE (days post-SE), blood and spleen smears stained with May-GrünwaldGiemsa as well as splenocytes cultures of 3DPSE showed an increase in relative abundance ofplasmocyte-like cells. Histological analysis of spleen sections showed increased cell density in thespleen white pulp and brain sections presented increased abundance of CD4+ lymphocytes in thechoroid plexus as well as CD4+infiltrating cells in brain parenchyma. Our results suggest thatperipheral immune system is probably responding to brain-derived clues released by the SE.Supported by PICT 2015-1451; UBACYT; and FONCYT fellowship (PS)