LA EXPRESIÓN DEL RECEPTOR ADENOSINA A1 ESTÁ MODIFICADO EN LA MÉDULA ESPINAL EN UN MODELO DE EPILEPSIA EXPERIMENTAL

PICHEL M; AUZMENDI J; GIRARDI E

Montevideo, Uruguay

Congreso; 5º Congreso Latinoamericano de Epilepsia.; 2008

La Liga Internacional contra la Epilepsia (ILAE) y el Buró Internacional para la Epilepsia (IBE)

The clinical signs associated with epilepsy have the origin in supraspinals structures, but it is the spinal cord responsible of generating the typical tonic clonic contractions associated with seizures. The spinal cord isolated from the brain has convulsive paroxystic motor activity, which is observed in intact animals. Adenosine is an endogenous neuromodulator with anticonvulsive properties and regulates the neuronal through CNS specific receptors. Adenosine is released in the CNS after seizures. In this work the distribution of adenosine A1 receptors in the spinal cord and the effect of the administration of the convulsant drug 3-mercaptopropiónico acid (MP) has been studied. Methods: Lots of Wistar rats (250-300g) were administered during seven days with daily doses of MP, 45mg/kg, i.p., (MP). Rats injected with saline were used as control. One day after last injection rats were anesthetized and perfused extracting the spinal cord of each region and processed for immunohistochemical assays using antiadenosine A1R as primary antibody and peroxidase- antiperoxidase techniques. Results: Immunohistochemical studies showed A1R expression in ventral horn in all regions. MP induces tonic-clonic seizures. After MP 7, A1R decreased 76% (D.O: 0.327 ± 0.004) respect to control in cervical region, 52% (D.O:0.229±0.006) thoracic area and 70% (D.O: 0.305±0.004) in lumbar area. Conclusions: The decreased expression of the receptor A1 in the model of MP repetitive convulsions suggests that adenosine would not be protecting from seizures.