Seizure-dependent regulation of Abcb1a and Abcb1b gene levels in the GASH/Sal model of audiogenic seizures

LAURA ZEBALLOS FERNÁNDEZ; DIAZ CASADO, E; AUZMENDI, JERÓNIMO; LAZAROWSKI, ALBERTO; DOLORES E. LÓPEZ

Medellin

Congreso; XII Congreso Latinoamericano de Epilepsia; 2022

International League Against Epilepsy

Purpose: To analyze the expression changes of the Abcb1a andAbcb1b genes in the GASH/Sal model (Genetic audiogenic seizures, Salamanca) in response to different experimental conditions. These genes encode P-glycoprotein. This study will provide information of the expression changesof both genes in response to seizures, and their possible epileptogenic role through changes in the depolarization threshold of neurons.Methodology: Gene expression changes in the epileptogenic nucleus (inferior colliculus) in three GASH/Sal groups were evaluated by RT-qPCR: GASH/Sal naïve, no stimulation; GASH/Sal stimulated 20 times in 10 days ('classical' stimulation); and GASH/Sal with 45 stimulations in 15 days.Results: Abcb1b levels in the inferior colliculus are similar when comparing the GASH/Sal naïve group with the GASH/Sal classical stimulation group, while Abcb1a levels decrease (1.164 ± 0.1775; 0.5782 ± 0.03044; p. < 0.0074 ). GASH/Sal hamsters with 45 stimulations show even lower expression of Abcb1a when compared to naïve hamsters (1.164 ± 0.1775; 0.1831 ± 0.03938; p.