Hydatid fluid from Echinococcus granulosus induces phagophore and autophagosome formation in dendritic cells through an upregulation of beclin-1

CHOP, MAIA; NICOLAO, MARÍA CELESTE; LOOS, JULIA A.; LEDO, CAMILA; CUMINO, ANDREA C.; RODRIGUEZ RODRIGUES, CHRISTIAN

Congreso; Reunión Anual de Sociedades de Biociencias (SAIC-SAI-AAFE-NANOMED.ar); 2021

Background: The cestode Echinococcus granulosus (Eg) is theetiological agent of cystic echinococcosis. This parasite developscysts filled with hydatid fluid (HF) in the viscera of the intermediatehost. Autophagy is a cellular catabolic process that plays a key rolein the presentation of endogenous and exogenous proteins, promotingthe activation of T cells. The aim of this work is to analyze if HF,constituted by a wide range of parasite proteins, could trigger autophagyin dendritic cells. Methods: HF was punctured from the hydatidcysts collected of infected cattle slaughtered. Murine BMDCswere cultured in RPMI 1640 medium, supplemented with FLT3-L.First, lysosome activity was evaluated using Acridine Orange, a fluorophorethat can be trapped in acidic vesicular organelles. Then,autophagy induction was evaluated by FACS, qPCR, Confocal andTransmission Electron Microscopy. Rapamycin (20 nM) and chloroquine(100μM) were used to modulate autophagic flux. LC3-attachmentto the autophagic membrane, were analyzed by stainedDCs with anti LC3-β antibody (clone H50). Results: HF significantlyincreased acridine orange cytoplasmic accumulation compared tocontrol cells (***p