Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T-cell proliferation

CHOP, MAIA; PLÁ, NATALIA; LOOS, JULIA A.; NICOLAO, MARÍA CELESTE; CUMINO, ANDREA C.; RODRIGUEZ RODRIGUES, CHRISTIAN

Virtual

Congreso; Reunión Anual de Sociedades de Biociencias (SAIC-SAI-SAFIS) 2020; 2020

SAIC-SAI-SAFIS

Background: Autophagy is an important process for the presentationof endogenous and exogenous proteins on MHC I and II molecules,promoting activation of CD8+ and CD4+ T cells respectively. Theaim of this work is to analyze if hydatid fluid (HF) from Echinococcus,constituted by a wide range of parasite proteins could trigger auto-phagy improving antigen presentation and T cell proliferation. Meth-ods: BMDCs were cultured in complete RPMI. Hydatid fluid (HF) waspunctured from the hydatid cysts collected of infected cattle slaugh-tered. Antigen uptake was measured with (FITC-OVA) in BMDCsusing a standard method. HF-stimulated BMDCs, were evaluated inautophagy induction and MHC II expression. For it, fixed cells wereimmunostained with LC3-b (clone H50) and analyzed them by im-munofluorescence confocal microscopy. CFSE-stained splenocyteswere co-incubated with BMDCs using a DC: splenocyte ratio of 1:4Cellular proliferation was assayed after 4 days of culture by flowcytometry. Results: First, we evaluated if stimulation of HF during 18h in BMDCs, induce different rates of antigen uptake. Effectively, thepresence of Echinococcus antigens induces a markedly decreasedOVA-uptake compared to control (**p