Hydatid fluid form E. granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro

CHOP, MAIA; LEDO, CAMILA; NICOLAO, MARÍA CELESTE; LOOS, JULIA A.; CUMINO, ANDREA C.; RODRIGUEZ RODRIGUES, CHRISTIAN

Mar del Plata

Congreso; LXVII Reunión Anual Sociedad Argentina de Investigación Clínica, LXX Reunión anual de la Sociedad Argentina de Inmunología (SAI), Reunión Anual de Sociedades de Biociencias (SAIC-SAI-FAIC-SAFIS); 2022

Background: Cystic echinococcosis is caused by Echinococcus granulosus. This parasite develops cysts in the host that are filled with hydatid fluid (HF). Autophagy plays a key role in antigen presentation promoting activation of T cells. The aim of this work is to analyze if HF could polarize the T cell response in vitro. Methods: HF was collected from infected cattle. Expression of Clec9a was evaluated by FACS in FLT3L-BMDCs. Autophagy induction was evaluated by FACS, qPCR, confocal and TEM. Rapamycin (20 nM) and chloroquine (100μM) were used to analyze autophagic flux. T cell cytokine profiles were analyzed by qPCR. Results: HF induces a downmodulation of Clec9a in BMDCs (n=3 **p