Anti-Müllerian hormone: how early can it be used as a biomarker for future in vitro embryo production in Bos taurus cattle?

JCL MOTTA; CARRANZA A; C RYKACZEWSKI; NP FOLCHINI; M SAAD; C HAYDEN; RV SALA; R BOND; DC PEREIRA; PJ ROSS; A GARCIA-GUERRA

Denver, Colorado

Encuentro; IETS 50th; 2024

IETS

Circulating anti-Müllerian hormone (AMH) in cattle is associated with antral follicle count and embryoyield after in vitro embryo production (IVEP). However, the ability of AMH determination early in life toserve as a predictor of future embryo yield remains unknown. The aims of this study, therefore, were toassess: i) changes in AMH from birth to 365 d and ii) the association between AMH determined atdifferent ages and IVEP outcomes at 365 d of age. Holstein heifers (n = 113) had plasma samplescollected from birth (0 d) to 308 d of age at 28 d intervals. At ~365 d (range 326 – 401) of age aplasma sample was collected, and heifers were submitted for IVEP. Briefly, follicular wave emergencewas synchronized using follicle ablation and an intravaginal P4 device (CIDR, Zoetis) was administered.Superstimulation consisting of four 50 mg administrations (12 h apart) of pFSH (Folltropin-V,Vetoquinol) were initiated 36 h later. Ovum pick-up (OPU) was performed 44 h after the last pFSHadministration and cumulus-oocyte complexes (COCs) were subjected to IVEP. Plasma samples wereassayed for AMH using a commercial assay (Bovine AMH ELISA, AnshLabs). Data were analyzed usingcorrelation analysis and generalized linear mixed models. Circulating AMH was affected by age (P0.05) correlated with embryo yield at 365 d, while AMH between 196 and308 d was weakly (P