Antimicrobial activity of indoleacetic, gibberellic and coumaric acidsagainst Paenibacillus larvae and its toxicity against Apis mellifera

SZAWARSKI N.; GIMÉNEZ-MARTÍNEZ PABLO; MITTON G.; NEGRI PEDRO; MEROI ARCERITO F.R.; MARÍA PAZ MOLINÉ; SANDRA FUSELLI; EGUARAS M.; LORENZO LAMATTINA; MAGGI M.

Spanish Journal of Agricultural Research

Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)

Año: 2020 vol. 18

Aim of study: To explore three isolated phytomolecules: indoleacetic acid (IAA), gibberellic acid (GA), and the secondary mebolite p-coumaric acid (CUM): (1) evaluating their toxicity against Apis mellifera larvae and adults under controlled conditions the laboratory; (2)  searching for antimicrobial activity against Paenibacillus larvae. Area of study: Honey bee larvae and adults were collected from the experimental apiary of the ?Centro de Investigación en Abejas sociales (CIAS)? (-37.9348798, -57.682817), Institute of the  National University of Mar del Plata (UNMdP), Argentina. Material and methods: Paenibacillus larvae strains were isolated from beehives from different provinces of Argentina (Buenos Aires, Córdoba and Entre Ríos) showing clinical symptoms of theAmerican foulbrood. All strains (S1, S2, S3, S4) were genotypically identified using PL5 and PL4 primers and characterized as genotype ERIC1. Then standard essays were performed to determined toxicity of phytomolecules in honey bees and antimicrobial activity through the broth microdilution method.  Main results: The diet with GA, IAA andCUM did not present toxic effects in larvae or adult bees, and only CUM showed anmicrobial activity against P. larvae. In this study, we obtained in vitro values of MNIC(minimum non-inhibitory concentration) 500 μg mL 1  and a MIC (minimum inhibitory concentration) of 650 μg mL -1  for CUM. Research highlights: The obtained results remark its potential as a natural alternative for the control of P. larvae, avoiding the problems generated by the use of synthetic antibiotics such as the resistance phenomena and the contamination of hive?s products.