INVESTIGADORES
YARYURA Pablo Marcelo
congresos y reuniones científicas
Título:
A NOVEL NTRC TRANSCRIPTION FACTOR REGULATES CHEMOTAXIS, BIOFILM FORMATION AND VIRULENCE IN XANTHOMONAS CITRI SUBSP. CITRI
Autor/es:
YARYURA PM; CONFORTE VP; MALAMUD F; ROESCHLIN R; DE PINO V; CASTAGNARO AP; DOW JM; MARANO MR; VOJNOV AA
Lugar:
Mar del Plata
Reunión:
Congreso; VIII Encuentro Latinoamericano y del Caribe de Biotecnología; 2013
Resumen:
Citrus
canker, caused by Xanthomonas citri
subsp. citri (Xcc), is one of the mayor diseases producing great economic losses
worldwide. Xcc ability to form
biofilm structures on leaves is closely related to its virulence. Through a screening
of mutants generated by the Tn5
transposon insertion in order to find those defective in biofilm formation, we
found the ntrC mutant. This gene
encodes a transcription factor that had not been described previously in Xcc.
Here
we demonstrated that the ntrC mutant
is unable to form the wild type mature biofim structures. Its capacity to move toward
grapefruit leaves extracts is severely reduced due to the absence of flagella
as a consequence of the down-regulation of flagellar genes. Moreover, this ntrC mutant is less virulent than the
wild type strain. Introduction of gene encoding an enzyme that can degrade
cyclic 3',5'-diguanylate (c-di-GMP) into the ntrC mutant restored chemotaxis, motility and virulence to wild
type levels. This remark suggests a possible relationship between NtrC and c-di-GMP
in bacteria.