PHOTOACOUSTIC CHARACTERIZATION OF NEW UV-MALDI MATRICES

DAVID GARA PEDRO; BILMES GABRIEL; PETROSELLI GABRIELA; ERRA BALSELLS ROSA

Merida

Congreso; 16 International Conference on Photoacoustic and Photothernal Phenomena; 2011

The knowledge of the thermal deactivation process of common UV-MALDI matrices should play a critical role in understanding why some matrices lead more abundant post-source decay as well as prompt decay of the analyte. Furthermore, it will be helpful in order to choose the proper matrix to optimize UV-MALDI-MS analysis. In this work the calorimetric behaviour and triplet state properties of a group of recently proposed matrices used in UV-MALDI-MS for lipids analysis were determined by performing photoacoustic measurements using nanosecond low energy laser pulses for excitation and piezoelectric detection. 9-aminoacridine (9AA), 3-aminoquinoline (3AQ), 2-(2-aminoethylamino)-5-nitropyridine (AAN) and 3,4-dihydro-7-methoxy-1-methyl-9H-pyrido[3,4b]indole(harmaline, Hla) were studied with this method in acetonitrile and methanol solutions Photoacoustic results were complemented with photostability, absorption, emission and singlet oxygen production measurements to characterize the photophysical, photochemical and spectroscopic behaviour of these molecules.