PATERNAL MODERATE ALCOHOL CONSUMPTION MODIFIES MOUSE PERI-IMPLANTATION EMBRYO DEVELOPMENT IMPAIRING INNER CELL MASS AND TROPHOBLAST GROWTH AND MORPHOLOGY

GOTFRYD L; FUENTES, FEDERICO; SANCHEZ M; SALAMONE G; CALVO JC; CEBRAL E; FONTANA V

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Previously, we showed that paternal alcohol intake partially inhibitssperm capacitation, increases nuclear decondensation rate andderegulates the dynamics of pronucleus formation and fertilization.Aim: To evaluate the effect of paternal alcohol consumption and itsconsequences on mouse early embryo development focusing ontrophoblast (TB) differentiation and inner cell mass (ICM) as criticalembryo stages invading maternal decidua. Methods: CF-1 fertilemales were exposed (treated group, T) or not (control group, C) to15% (v/v) ethanol in drinking water ad libitum for 15 days. They weremated with a fertile nontreated CF-1 female (1:1). Positive mating femaleswere sacrificed at day 2 of gestation to obtain 2 cell embryoswhich, then, were cultured for 7 days. Embryo differentiation, growthand morphology were evaluated during pre- and peri-implantationphases in vitro. Then, embryos were classified as type A (ICM: protrudingaggregates of compact cells; TB: symmetric monolayer offlat and elongated cells) or type B (ICM: disaggregated, few scatteredor no cells, TB: asymmetric trophoblast outgrowth). Frequencydifferences between C and T were tested by Fisher´s exact test.Results: Male alcohol consumption for 15 days delayed the embryodifferentiation and growth by detention/fragmentation and alteredthe embryo morphology. Differences between type A and B distributionin C and T were statistically significant for ICM and TB in bothgroups. For ICM: Type A 53% C vs 10% T (p