Proteome analysis of Bordetella pertussis isolated from human macrophages

LAMBERTI Y; CAFIERO, HILARIO; SURMANN, KRISTIN; VALDEZ H; HOLUBOVA, JANA; VECEREK, BRANISLAV; SEBO, PETER; SCHMIDT, FRANK; VÖLKER, UWE; RODRIGUEZ, MARÍA EUGENIA

JOURNAL OF PROTEOMICS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 p. 55 - 67

1874-3919

Previous studies have shown that B. pertussis survives inside human macrophages in non-acidic compartments with characteristics of early endosomes. In order to gain new insight into the biology of B. pertussis survival in host cells, we have analyzed the adaptation of the bacterial proteome during intracellular infection. The proteome of B. pertussis 3 h and 48 h after infection of human macrophage-like THP-1 cells was examined by nano-liquid chromatography combined with tandem MS and compared to the protein profile of extracellular B. pertussis growing in the same cell culture medium. Compared with extracellular bacteria, almost 300 proteins out of 762 identified proteins displayed altered levels in intracellular B. pertussis. Functional analyses of the proteins displaying altered abundance revealed enrichment of proteins involved in stress response, iron uptake, cellular metabolism, transcriptional regulation, and virulence.To our knowledge, this is the first analysis of the B. pertussis proteome during adaptation to the intramacrophage environment and the data provide new clues for understanding B. pertussis adaptation and pathogenesis.