Barcodes in GF(4): Multiplexing for upcoming 3G sequencing technology

TAPIA ELIZABETH; BULACIO PILAR; SPETALE FLAVIO EZEQUIEL; ANGELONE LAURA; ORNELLA LEONARDO

Florianópolis

Conferencia; 7TH Conference of the Brazilian association for BioInformatics and computational Biology; 2011

Molecular barcoding provides an opportunity to spread, i.e., tomultiplex, NGS capacity across multiple individuals at specificportions of the genomes, thus providing a better compromisebetween cost, coverage and throughput for populationgenomics projects. Molecular barcoding lays on the ability ofshort oligos, known as barcodes, to tag DNA fragmentsbelonging to different samples. Despite the deploymentmethod, barcodes must assure both resilience againstsequencing errors and minimum interference with DNAsequencing reactions.Current design of barcodes rely on exhaustive searches of thespace of oligo, thus limiting barcoding applications to tens ofsamples and low sequencing error rates. Considering thatupcoming 3rd generation (3G) sequencing technologies willgive us improved read lengths but also higher sequencing errorrates, it follows that the problem of designing barcodingschemes with improved multiplexing and error resiliencecapacities deserves special attention