Protective effect of naringin against bone alterations caused by the metabolic syndrome and environmental stress

RODRIGUEZ VALERIA; RIVOIRA MARÍA ANGÉLICA; RIVAROLA MARÍA ANGELICA; PICOTTO GABRIELA; DIAZ DE BARBOZA GABRIELA; BRUN LUCAS; MIR F; SUAREZ MARTA; TOLOSA DE TALAMONI, NORI

Congreso; Congreso SAIC; 2022

We evaluated the effect of Naringin (NAR) on the alterations in long bones caused by a high-fructose diet (HFD) in rats exposed or not to environmental chronic stress (CS). Male Wistar rats were divided: 1) Rats with metabolic syndrome (MS): fed 10% HFD (w/v) in drinking water for 30 days, 2) MS rats exposed to CS, 3) MS rats treated with NAR (40 mg NAR/kg b.w.) and 4) rats with MS and CS treated with NAR. CS was variable, random, and unpredictable. Systemic biochemical parameters, body weight and waist circumference were measured. Histomorphometric parameters were measured in the proximal tibia. Bone mineral density (BMD) was determined in femur and tibia. Oxidative stress in femur bone marrow was evaluated. Adipocytes and osteocytes were counted in tibia slices. ANOVA/Bonferroni was used for statistical analysis. MS+CS rats had lower body weight and higher serum corticosterone levels compared to MS rats; NAR treatment normalized these parameters. Serum osteocalcin and proximal tibial bone volume were lower in MS rats compared to the other groups. MS+CS rats showed an increase in trabecular separation. NAR treatment increased trabecular thickness in both MS and MS+CS rats, and prevented increased trabecular separation in MS+CS rats. Tibia BMD of MS rats was lower than that of the other groups. NAR increased femur BMD in MS and MS+CS rats. Stress increased the number of adipocytes in tibias of MS rats. NAR decreased adipocytes in MS rats and in MS+CS rats. The number of osteocytes was lower in MS rats compared to the other groups. In bone marrow of MS+CS rats, total GSH content was lower than that of MS rats. NAR increased total GSH and decreased ˙O2− content in MS and MS+CS rats. Catalase activity in MS rats was lower compared to the other groups. In conclusion, NAR treatment partially prevents bone deterioration and oxidative stress in bone marrow caused by MS in the absence and presence of CS.